Expediting in Vitro Characterization of mRNA-Based Gene Therapies via High-Content Fluorescent Imaging


Authors: T.N. Vigila, D. Zhang-Hulsey, J.L. Santos and G.P. Hussmann

Journal: Analytical Biochemistry

DOI: https://doi.org/10.1016/j.ab.2021.114259

Publication - Abstract

August 15, 2021

mRNA Nucleic Acid Lipid Nanoparticles
A study conducted by Vigil et al. 2021 from Astrazeneca Biopharmaceuticals R&D showcases the applicability of high-content fluorescent imaging as a technique for the rapid characterization of mRNA-LNPs generated from a NanoAssemblr® mixing device in order to determine the optimal conditions for transfection that minimize cellular cytotoxicity and maximize mRNA internalization. Through high-content fluorescent imaging, mRNA-LNP characteristics such as transfection parameters, transfection-induced cytotoxicity, mRNA delivery, and mRNA translation were investigated in HEPG2 two dimensional monolayer and three dimensional spheroid cell cultures. This research demonstrates the value in utilizing high fluorescent imaging especially for future studies toward the development of novel gene therapies and drug candidates for various disease areas.

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