Modified mRNA-Based Vaccines Elicit Robust Immune Responses and Protect Guinea Pigs From Ebola Virus Disease


Authors: M. Meyer, E. Huang, O. Yuzhakov, P. Ramanathan, G. Ciaramella and A. Bukreyev

Journal: Journal of Infectious Diseases

DOI: 10.1093/infdis/jix592

Publication - Abstract

December 21, 2017

Vaccines Nucleic Acid Lipid Nanoparticles mRNA

Abstract

Most current Ebola virus (EBOV) vaccine candidates are based on viral vectors, some of which cause side effects or require complex manufacturing. Modified mRNA vaccines are easily produced, safe, and are highly immunogenic. We developed 2 mRNA vaccines based on the EBOV envelope glycoprotein, which differed by the nature of signal peptide for improved glycoprotein post-translational translocation. The mRNAs were formulated with lipid nanoparticles to facilitate delivery. Vaccination of guinea pigs induced EBOV-specific IgG and neutralizing antibody responses and 100% survival after EBOV infection. The efficacy of our mRNA vaccine combined with preclinical safety data supports testing in clinical studies.


limit search to this category
Advanced Search

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Abstract

Jun 15, 2018
Molecular therapy

Lipid Nanoparticle-Delivered Chemically Modified mRNA Restores Chloride Secretion in Cystic Fibrosis

E. Robinson, K.D. MacDonald, K. Slaughter and M. McKinney

mRNA Nucleic Acid Lipid Nanoparticles Genetic Medicine
The promise of gene therapy for the treatment of cystic fibrosis has yet to be fully clinically realized despite years of effort toward correcting the underlying genetic defect in the cystic fibrosis transmembrane conductance regulator (CFTR).
Read More


Publication - Abstract

Oct 29, 2020
Journal of Medicinal Chemistry

Property-Driven Design and Development of Lipids for Efficient Delivery of siRNA

K. Rajuppan, S.P. Tanis, R. Mukthavaram, S. Roberts, M. Nguyen, K. Tachikawa, P. Karmali et. al.

siRNA Other Applications Nucleic Acid Lipid Nanoparticles
Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Cytiva, formerly Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.
Sign Up