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Liposomes
Liposomes are drug delivery vehicles that can be formulated with a wide variety of natural, synthetic, and modified lipid species to deliver drugs and contrast agents. There are more than 15 liposomal drug
formulations on the market for indications such as cancer, fungal infections, macular degeneration, pain management and vaccines. There are many applications using liposomes in clinical, preclinical, and basic research, including:
• Drug delivery
• Gene delivery/transfection
• Contrast agents for medical imaging (eg: ultrasound, MRI
and fluorescence)
• Precursors for lipid coatings (eg: biosensors)
Overcome Key Challenges in Advancing Liposome Formulations
| Challenges with conventional methods | Solutions with the NanoAssemblr Platform | |
| The formulation process has significant batch-to-batch variability |  | Reproducible liposome manufacturing conditions |
| Maintaining precise control over the particle size is difficult | | Control liposome size through instrument parameters |
| Loading liposomes requires multiple steps | | Combined drug loading and liposome formation in one step for efficient and versatile drug loading |
| Production is time-consuming and labor-intensive | | Rapid, effortless liposome production and optimization |
| The manufacturing process is difficult to scale-up | | A seamless path to scaling up production |
Reproducible Liposome Manufacturing Conditions
Liposome size and polydispersity were consistent across six independent batches prepared by different operators.Liposome Size Control Through Convenient Instrument Parameters
The effects of adjusting Total Flow Rate (TFR) and Flow Rate Ratio (FRR) in the NanoAssemblr® software on nanoparticle size. Parameters can be easily adjusted to achieve a specific size for a given lipid composition.
Combine Drug Loading and Liposome Formation in One Step for Efficient and Versatile Drug Loading
Encapsulation efficiency of verteporfin loaded liposomes containing natural (egg-PC, soy-PC) or synthetic phospholipids (POPC, DSPC) as the primary lipid component.
A Seamless Path to Scaling Up Production
An example shown here is a liposome formulation (POPC/Cholesterol 60/40 mol/mol), optimized using the NanoAssemblr Benchtop and scaled up using the NanoAssemblr® Blaze™ and the GMP System with minimal process development and identical results.
An organic solvent with dissolved lipids and an aqueous solution are injected into the two inlets of the NanoAssemblr® mixer.
With laminar flow, the two solutions do not immediately mix. Microscopic features engineered into the channels cause the two fluids to intermingle in a controlled and reproducible way. Within 1 ms, the two fluids are completely mixed. This mixing
causes a change in solvent polarity which triggers self-assembly of lipids into liposomes. Controlling the speed and ratio of fluid injection controls the size of the liposomes.
Liposome Resources
Publication - Abstract
May 08, 2020
Vaccines
Publication - Abstract
April 02, 2020
Journal of Controlled Release
Publication - Abstract
September 15, 2021
Vaccine
Publication - Abstract
March 12, 2021
Pharmaceutics
Publication - Abstract
December 16, 2020
pharmaceutics
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