Preparation and Optimization of Lipid-Like Nanoparticles for mRNA Delivery


Authors: B. Li and Y. Dong

Journal: Methods in Molecular Biology

DOI: 10.1007/978-1-4939-7138-1_13

Publication - Abstract

July 21, 2017

mRNA Nucleic Acid Lipid Nanoparticles Genetic Medicine

Abstract:

Lipid-like nanoparticles (LLNs) have shown great promise for nucleic acid delivery. Recently, we have developed N1,N3,N5-tris(2-aminoethyl)benzene-1,3,5-tricarboxamide (TT) derived lipid-like compounds, formulated them into TT LLNs for mRNA delivery, and applied an orthogonal array design to facilitate formulation optimization. This chapter focuses on the following contents relevant to lipid-like nanoparticles: formulation method, particle characterization, orthogonal array design, and in vitro assays.


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