Enhancing Inflammation Targeting Using Tunable Leukocyte-Based Biomimetic Nanoparticles


Authors: A. Zinger, M. Sushnitha, T. Naoi, G. Baudo, E. De Rosa, J. Chang, E. Tasciotti and F. Taraballi

Journal: ACS Nano

DOI: 10.1021/acsnano.0c05792

Publication - Abstract

March 16, 2021

Other Payloads Targeted Drug Delivery Other Applications

Abstract

Biomimetic nanoparticles aim to effectively emulate the behavior of either cells or exosomes. Leukocyte-based biomimetic nanoparticles, for instance, incorporate cell membrane proteins to transfer the natural tropism of leukocytes to the final delivery platform. However, tuning the protein integration can affect the in vivo behavior of these nanoparticles and alter their efficacy. Here we show that, while increasing the protein:lipid ratio to a maximum of 1:20 (w/w) maintained the nanoparticle’s structural properties, increasing protein content resulted in improved targeting of inflamed endothelium in two different animal models. Our combined use of a microfluidic, bottom-up approach and tuning of a key synthesis parameter enabled the synthesis of reproducible, enhanced biomimetic nanoparticles that have the potential to improve the treatment of inflammatory-based conditions through targeted nanodelivery.


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